| production method | extract 150g of acetone powder for posterior pituitary gland of pig brain, add 375ml of acetone, stir, add 2400ml of glacial acetic acid, heat for half an hour in a water bath at 70 ℃, cool to below 35 ℃, filter, wash the filter residue with 300ml of glacial acetic acid and 300ml of acetone respectively, and combine washing and filtrate to obtain the extract. Porcine posterior pituitary acetone powder [acetone; Glacial acetic acid] →[70 ℃] Separation and precipitation of extract 14ml of saturated NaCl solution is added to the extract, 1390ml of acetone is added to precipitate the precipitation, refrigerated overnight, filtered the next day to obtain 4000ml of filtrate. If it is not clarified, it can be filtered by No. 4 vertical melting funnel, and prolactin is recovered from the filter residue. Add the same volume of ether under stirring, let it stand for a while, absorb the supernatant, filter, precipitate with acetone to wash off acetic acid, then wash with anhydrous ether twice, and dry with phosphorus pentoxide to obtain crude product. Extraction solution [saturated NaCl, acetone] → filtrate [diethyl ether] → MSH crude refined crude product 22g is dissolved in 620ml acetic acid (0.1mol/L), 1 mol/L hydrochloric acid, water and 3.8g of oxidized cellulose washed with acetic acid (0.1mol/L) are added, stirred for 4h, and filtered in No. 3 vertical melting funnel to obtain recycled oxidized cellulose. Wash with the aforementioned acetic acid for 3-4 times, wash with distilled water for several times, place in a beaker after suction and filtration, add 82ml of acetic acid (the same concentration as before) and stir for half an hour. After filtration, wash with 0.1mol/L hydrochloric acid 82ml stirring once, and combine filtrate. The pH of ammonia water is adjusted to 6.5, the supernatant is collected by centrifugation, 1L of cold acetone is added for refrigeration, centrifugation, the collection precipitate is washed twice with acetone, once with ether, and the phosphorus pentoxide dryer is dried to obtain refined products. MSH crude [acetic acid, oxidized cellulose, HCl]→ [ammonia, acetone, pH6.5] finished product. |